The hemicellulases from the ethanologenic thermophile: Themoanaerobacter ethanolius

Technical Report · Wed May 01 04:00:00 EDT 1991

DOI:https://doi.org/10.2172/5012379· OSTI ID:5012379

Wiegel, J

Previously, we had only obtained extremely low xylanase activity in cultures of {ital Thermoanaerobacter ethanolicus} strain JW200, despite demonstrated xylan hydrolysis. We were not able to increase the enzyme activity or concentrate it in solution. Therefore, we decided to isolate new strains of thermophilic anaerobes with higher xylanase activity as a future source for cloning xylanases into {ital T. ethanolicus}. We now have organisms exhibiting 100-fold higher xylanase activity than JW200, but still cannot isolate or concentrate the enzyme activity except at very low yields. We have concentrated and partially purified a xylanase from strain N.D. using preparative matrix-free isoelectric focusing. We have also purified to homogeneity and partially characterized a xylosidase from {ital T. ethanolicus}. We have detected and measured arabinosidase and acetyl esterase activity in {ital T.ethanolicus}, {ital Clostridium thermohydrosulfuricum} and strain N.D. 7 refs., 2 tabs. (MHB)

• Research Organization: Georgia Univ., Athens, GA (United States). Research Foundation
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG09-89ER14059
• OSTI ID: 5012379
• Report Number(s): DOE/ER/14059-1; ON: DE92001859
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANAEROBIC CONDITIONS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOCONVERSION
BIOLOGY
BIOSYNTHESIS
CARBOHYDRATES
DOCUMENT TYPES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
ESTERASES
GENE REGULATION
GLYCOSYL HYDROLASES
HEMICELLULOSE
HYDROLASES
KINETICS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
OPTIMIZATION
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROGRESS REPORT
PURIFICATION
REACTION KINETICS
SACCHARIDES
SYNTHESIS
TAXONOMY
THERMOPHILIC CONDITIONS
XYLANASE