Effects of bisulfite on metabolic development in synchronous Chlorella pyrenoidosa

Journal Article · Tue Jan 01 04:00:00 EST 1974 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(74)90036-X· OSTI ID:5011184

Das, G; Runeckles, V C

Addition of bisulfite to synchronous cultures of Chlorella pyrenoidosa adversely affects both active growth and cell propagation. Analyses of chlorophyll, protein, RNA, and DNA revealed decreases of all these components with the decline in cell population, as HSO/sub 3//sup -/ concentration was increased. Biochemical analysis after treatment showed a differential change in the levels of chlorophylls, protein, RNA, and DNA, when expressed as percentages of the cell dry weight: the content of chlorophyll b increased, whereas that of chlorophyll a decreased gradually with increases in HSO/sub 3//sup -/. The amounts of protein and RNA per unit dry weight remained constant, but DNA levels were markedly reduced. With the exception of iron, the levels of nutrient elements within the cells increased with increasing HSO/sub 3//sup -/ concentration. The relative increases were most marked with K, Mg, Ca, Cu, and Na. The results suggest that HSO/sub 3//sup -/ adversely affects cell development by impairing DNA synthesis. 24 references, 5 figures, 2 tables.

• Research Organization: Univ. of British Columbia, Vancouver
• OSTI ID: 5011184
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 7; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGAE
ALKALI METALS
ALKALINE EARTH METALS
BIOLOGICAL EFFECTS
CALCIUM
CARBOXYLIC ACIDS
CHEMICAL COMPOSITION
CHLORELLA
CHLOROPHYLL
COPPER
DNA
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
GROWTH
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAGNESIUM
METALS
MICROORGANISMS
NUCLEIC ACIDS
NUTRIENTS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PLANT GROWTH
PLANTS
PORPHYRINS
POTASSIUM
PROTEINS
RNA
SODIUM
SULFITES
SULFUR COMPOUNDS
TRANSITION ELEMENTS
UNICELLULAR ALGAE