In vivo metabolism, disposition, and macromolecular binding of 1-nitro (/sup 14/C) pyrene vapor-coated onto diesel particles
The potent mutagen and environmental pollutant 1-nitropyrene (NP) labeled with /sup 14/C and vapour-phase-coated onto diesel particles (/sup 14/C-NP-DP; 380 ppm NP; 0.85 microCi/mg particles) was administered to rats (5 mg each) by oral or by intratracheal instillation. 6-Hydroxy-N-acetyl-1-aminopyrene was previously found to be the metabolite responsible for the majority of the mutagenic activity in the urines of rats injected with pure NP. Phenols of NP itself were also identified, both free and conjugated, representing 1-5% of the urinary /sup 14/C. The extent of urinary excretion indicated that NP was readily bioavailable from the dose remained in the lung 24 h after intratracheal instillation, and 5 to 15% of that /sup 14/C appeared to be bound to protein. Since NP has previously been shown to bind to lung DNA and protein in vitro, these results indicate that this organ might be particularly exposed in vivo and therefore vulnerable to possible genotoxic damage by NP.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
- OSTI ID:
- 5010895
- Report Number(s):
- PB-85-218857/XAB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDITIVES
AIR POLLUTION
CARBON 14 COMPOUNDS
CHEMICAL BONDS
DIESEL FUELS
LABELLED COMPOUNDS
LABORATORY ANIMALS
METABOLISM
MUTAGENS
PARTICLES
PETROLEUM PRODUCTS
POLLUTION
PUBLIC HEALTH
TOXICITY