Bench-scale evaluation of alternative biological treatment processes for the remediation of pentachlorophenol- and creosote-contaminated materials: Slurry-phase bioremediation

Journal Article · Sat Jun 01 04:00:00 EDT 1991 · Environmental Science and Technology; (United States)

DOI:https://doi.org/10.1021/es00018a006· OSTI ID:5003698

Mueller, J G; Lantz, S E ^[1]; Blattmann, B O ^[2]; Chapman, P J ^[3]

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1. Southern Bio Products, Inc., Gulf Breeze, Fl (United States)
2. Technical Resources, Inc., Gulf Breeze, FL (United States)
3. Environmental Protection Agency, Gulf Breeze, FL (United States)

Performance data on slurry-phase bioremediation of pentachlorophenol- (PCP-) and creosote-contaminated sediment and surface soil were generated at the bench-scale level. Aqueous slurries were prepared from sediment and surface soil freshly obtained from the American Creosote Works Superfund site at Pensacola, FL. Slurries (1.1 L) were incubated for 30 days in separate, 1.5-L bioreactors operated in the batch mode at 28.5C with continuous mixing (300 rpm), DO = 90% and pH = 7.0. Samples removed with time from each reactor were extracted and analyzed by gas chromatography for PCP and 42 monitored creosote constituents to delineate the activity of indigenous microorganisms. Changes in microbial biomass were also recorded. Excluding PCP, benzo(b)fluoranthene, benzo(k)fluoranthene, and indeno(1,2,3-cd)pyrene, slurry-phase bioremediation of highly contaminated sediment (pH adjusted) resulted in rapid and extensive biodegradation (3-5 days to biodegrade > 50% of targeted compounds) of monitored constituents. Conversely, microbial activity in surface soil slurries was slower and generally confined to the more readily biodegradable, lower molecular weight compounds. These data suggest that slurry-phase bioremediation strategies can be effectively employed to remediate creosote-contaminated materials.

• OSTI ID: 5003698
• Journal Information: Environmental Science and Technology; (United States), Journal Name: Environmental Science and Technology; (United States) Vol. 25:6; ISSN ESTHA; ISSN 0013-936X
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
54 ENVIRONMENTAL SCIENCES
540220* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
AROMATICS
BENCH-SCALE EXPERIMENTS
BIODEGRADATION
BIOMASS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
CHROMATOGRAPHY
CLEANING
COMPARATIVE EVALUATIONS
CONTROL
CREOSOTE
DECOMPOSITION
DECONTAMINATION
DERIVATIZATION
DIAGRAMS
DISPERSIONS
ENERGY SOURCES
EVALUATION
EXTRACTION
FERTILIZERS
GAS CHROMATOGRAPHY
GROUND WATER
HALOGENATED AROMATIC HYDROCARBONS
HYDRIDES
HYDROCARBONS
HYDROGEN COMPOUNDS
LAWS
MICROORGANISMS
MIXTURES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC SILICON COMPOUNDS
OXYGEN COMPOUNDS
POLLUTANTS
POLLUTION LAWS
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
QUALITY ASSURANCE
QUALITY CONTROL
QUANTITATIVE CHEMICAL ANALYSIS
REMEDIAL ACTION
RENEWABLE ENERGY SOURCES
SEDIMENTS
SEPARATION PROCESSES
SILANES
SILICON COMPOUNDS
SLURRIES
SOILS
SOLVENT EXTRACTION
SUSPENSIONS
US SUPERFUND
WATER