Signals involved in T cell activation. II. Distinct roles of intact accessory cells, phorbol esters, and interleukin 1 in activation and cell cycle progression of resting T lymphocytes
Journal Article
·
· J. Immunol.; (United States)
OSTI ID:5003034
The signals involved in the initiation of mitogen-induced activation of resting guinea pig T cells were examined. The combination of phytohemagglutinin (PHA) and 4..beta..-phorbol 12-myristate 13-acetate (PMA) stimulated DNA synthesis by accessory cell (AC)-depleted T cells cultured at high density, but the use of low density cultures indicated that intact AC were absolutely necessary for PHA-stimulated T cell DNA synthesis even in the presence of PMA, interleukin 1 (IL 1), or interleukin 2 (IL 2). In contrast, AC-depleted T cells were able to respond to the combination of the calcium ionophore, ionomycin, and PMA regardless of the cell density at which they were cultured. Results of cell cycle analysis support the conclusion that intact AC, IL 1, and a PMA-like signal play distinct roles in the progression of mitogen stimulated T cells through the first round of the cell cycle.
- Research Organization:
- Southwestern Medical School, Dallas, TX
- OSTI ID:
- 5003034
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:10; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Activation of resting T cells: distinct roles of intact accessory cells, phorbol myristate acetate and interleukin 1
Signals involved in T cell activation. I. Phorbol esters enhance responsiveness but cannot replace intact accessory cells in the induction of mitogen-stimulated T cell proliferation
Early precursor thymocytes can produce interleukin 2 upon stimulation with calcium ionophore and phorbol ester
Conference
·
Tue Mar 04 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6787348
Signals involved in T cell activation. I. Phorbol esters enhance responsiveness but cannot replace intact accessory cells in the induction of mitogen-stimulated T cell proliferation
Journal Article
·
Thu Oct 31 23:00:00 EST 1985
· J. Immunol.; (United States)
·
OSTI ID:6935705
Early precursor thymocytes can produce interleukin 2 upon stimulation with calcium ionophore and phorbol ester
Journal Article
·
Fri Feb 28 23:00:00 EST 1986
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:5714495
Related Subjects
550300 -- Cytology
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CELL CYCLE
CHEMICAL ACTIVATION
CONNECTIVE TISSUE CELLS
DNA REPLICATION
ESTERS
GUINEA PIGS
HEMAGGLUTININS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MITOGENS
MUCOPROTEINS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CELL CYCLE
CHEMICAL ACTIVATION
CONNECTIVE TISSUE CELLS
DNA REPLICATION
ESTERS
GUINEA PIGS
HEMAGGLUTININS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MITOGENS
MUCOPROTEINS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES