Development of phylogenetic probes specific to Burkholderia cepacia G4
- Michigan State Univ., East Lansing, MI (United States). Center for Microbial Ecology
Burkholderia (formerly Pseudomonas) cepacia G4 is capable of oxidizing trichloroethylene (TCE) while growing on toluene or phenol and has potential for use in remediating TCE-contaminated groundwater. To track G4 in the environment, polymerase chain reaction (PCR)-based phylogenetic probes were developed. Four sets of specific primers for PCR amplification of 16S ribosomal RNA gene sequences were designed and tested against both closely and distantly related environmental isolates. Two sets of the primers specifically detected the species B. cepacia, and the other two sets specifically detected the strain G4. The sensitivity of these primer sets was evaluated using DNA isolated from the G4 pure culture and form the sterile soils seeded with a known number of G4 and E. coli cells. These primer sets were able to detect 1 fg to 1 pg of template DNA rom the pure culture, and 2.1 {times} 10{sup 2} to 2.1 {times} 10{sup 4} G4 cells per g soil in the presence of 1.56 {times} 10{sup 8} E. coli cells. With such specificity and sensitivity, these probe could be useful in tracking B. cepacia G4 and its relatives in the environment.
- Sponsoring Organization:
- National Science Foundation, Washington, DC (United States); National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States)
- OSTI ID:
- 492232
- Report Number(s):
- CONF-950483--; ISBN 1-57477-006-3
- Country of Publication:
- United States
- Language:
- English
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