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Title: Identification of the primary mechanism for fungal lignin degradation. Progress report

Abstract

Many lignin-degrading fungi appear to lack lignin peroxidase (LiP), an enzyme generally thought important for fungal ligninolysis. The authors are working with one of these fungi, Ceriporiopsis subvermispora, an aggressive white-rotter that selectively removes lignin from wood. During this project period, they have obtained the following principal results: new polymeric lignin model compounds were developed to assist in the elucidation of fungal ligninolytic mechanisms; experiments with one of the polymeric lignin models showed that C. subvermispora cultures which express no detectable LiP activity are nevertheless able to degrade nonphenolic lignin structures, this result is significant because LiPs were previously considered essential for fungal attack on these recalcitrant structures, which constitute about 90% of lignin; manganese peroxidases (MnPs), which C. subvermispora does produce, catalyze the peroxidation of unsaturated fatty acids to give fatty acid hydroperoxides, fatty acid hydroperoxides are also used by MnP as oxidants (in place of H{sub 2}O{sub 2}) that support the MnP catalytic cycle, these results indicate that MnP turnover in the presence of unsaturated lipids generates reactive lipid oxyradicals that could act as oxidant of other molecules; MnP-mediated lipid peroxidation results in the co-oxidative cleavage of nonphenolic lignin structures, the MnP/lipid peroxidation system may therefore provide C.more » subvermispora and other LiP-negative fungi with a mechanism to degrade the principal structures of lignin.« less

Publication Date:
Research Org.:
Wisconsin Univ., Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Energy Research, Washington, DC (United States)
OSTI Identifier:
491510
Report Number(s):
DOE/ER/20140-1
ON: DE97006353; TRN: AHC29714%%48
DOE Contract Number:  
FG02-94ER20140
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1997]
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; LIGNIN; FUNGI; ENZYMATIC HYDROLYSIS; PROGRESS REPORT; ENZYME ACTIVITY; PEROXIDASES; BIOMASS; WOOD

Citation Formats

NONE. Identification of the primary mechanism for fungal lignin degradation. Progress report. United States: N. p., 1997. Web. doi:10.2172/491510.
NONE. Identification of the primary mechanism for fungal lignin degradation. Progress report. United States. doi:10.2172/491510.
NONE. Sun . "Identification of the primary mechanism for fungal lignin degradation. Progress report". United States. doi:10.2172/491510. https://www.osti.gov/servlets/purl/491510.
@article{osti_491510,
title = {Identification of the primary mechanism for fungal lignin degradation. Progress report},
author = {NONE},
abstractNote = {Many lignin-degrading fungi appear to lack lignin peroxidase (LiP), an enzyme generally thought important for fungal ligninolysis. The authors are working with one of these fungi, Ceriporiopsis subvermispora, an aggressive white-rotter that selectively removes lignin from wood. During this project period, they have obtained the following principal results: new polymeric lignin model compounds were developed to assist in the elucidation of fungal ligninolytic mechanisms; experiments with one of the polymeric lignin models showed that C. subvermispora cultures which express no detectable LiP activity are nevertheless able to degrade nonphenolic lignin structures, this result is significant because LiPs were previously considered essential for fungal attack on these recalcitrant structures, which constitute about 90% of lignin; manganese peroxidases (MnPs), which C. subvermispora does produce, catalyze the peroxidation of unsaturated fatty acids to give fatty acid hydroperoxides, fatty acid hydroperoxides are also used by MnP as oxidants (in place of H{sub 2}O{sub 2}) that support the MnP catalytic cycle, these results indicate that MnP turnover in the presence of unsaturated lipids generates reactive lipid oxyradicals that could act as oxidant of other molecules; MnP-mediated lipid peroxidation results in the co-oxidative cleavage of nonphenolic lignin structures, the MnP/lipid peroxidation system may therefore provide C. subvermispora and other LiP-negative fungi with a mechanism to degrade the principal structures of lignin.},
doi = {10.2172/491510},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {6}
}