Concepts for the bioremediation of trinitrotoluene-contaminated wastes by the white rot fungus Phanerochaete chrysosporium
- Utah State Univ., Logan, UT (United States). Biotechnology Center
Biodegradation of trinitrotoluene (TNT) by the white rot fungus Phanerochaete chrysosporium was studied in both liquid reactors and soil microcosms. It has been previously shown that TNT mineralization by P. chrysosporium only occurs under lignin-degrading conditions. In this study TNT biotransformation rates were dependent on initial TNT concentration and the amount of fungal mycelia. Non-ligninolytic reactors containing 50 g/L mycelia were able to degrade up to 100 parts per million (ppm) TNT within four hours but the reduced aminodinitrotoluene (AmDNT) and diaminonitrotoluene (DAT) metabolites were not degraded, even after 70 days of incubation. On the other hand, ligninolytic reactors containing 8 g/L mycelia required 6 days to degrade 100 ppm TNT, and the AmDNT and DAT were completely degraded within 10 days. Ligninolytic conditions could be maintained for 18 days by addition of 3.0 g/L glucose and 0.25 mM ammonia to the reactors every three days. In soil, P. chrysosporium was able to metabolize 200 and 2,000 mg/kg TNT to less than 10 mg/kg within 14 and 100 days, respectively.
- OSTI ID:
- 491045
- Report Number(s):
- CONF-9406250--
- Country of Publication:
- United States
- Language:
- English
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