Biodegradation kinetics and bioreactor strategy for aromatic hydrocarbon and trichloroethylene mixtures

Conference · Sat Dec 31 04:00:00 EST 1994

OSTI ID:491019

Reardon, K F; Conuel, J R; Mosteller, D C ^[1]

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1. Colorado State Univ., Fort Collins, CO (United States). Dept. of Chemical and Bioresource Engineering

Although most contaminated sites contain a mixture of pollutants, little is known about the biodegradation of mixtures. Highlights of two investigations into mixture biodegradation are reported here. In the first, the kinetics of biodegradation of benzene, toluene and phenol by Pseudomonas putida F1 were measured in suspended-cell, batch cultivations, both individually and as mixtures. Significant substrate interactions were observed. A mathematical model that incorporates an interaction coefficient was applied to the data and was found to predict the kinetics well. In the second example, an immobilized-cell bioreactor for the cometabolic removal of trichloroethylene was developed. Pulsed feeding of the growth substrate, toluene, was found to remove trichloroethylene at a rate 35% higher than the removal rate with constant toluene feeding.

• Sponsoring Organization: National Insts. of Health, Bethesda, MD (United States)
• OSTI ID: 491019
• Report Number(s): CONF-9406250--
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
BENZENE
BIODEGRADATION
BIOREACTORS
CHEMICAL REACTION KINETICS
CHLORINATED ALIPHATIC HYDROCARBONS
DATA
IMMOBILIZED CELLS
MATHEMATICAL MODELS
PHENOL
REMEDIAL ACTION
TOLUENE