Aerobic biotransformation and mineralization of 2,4,6-trinitrotoluene
- Texas A and M Univ., College Station, TX (United States). Dept. of Civil Engineering
Respirometric mineralization studies of 2,4,6-trinitrotoluene (TNT) were conducted with microorganisms isolated from a site contaminated with munitions waste in Illinois. Nine aerobic bacterial species were isolated under a carbon- and nitrogen-limited condition and tentatively identified as: one Pseudomonas species; one Enterobacter species; and seven Alcaligenes species. Experiments were performed using each of the nine organisms individually and with a consortium of all nine bacterial species. The aerobic microorganisms were cultured in a sterile nutrient solution with glucose and 20 mg/L TNT. Mineralization was determined using uniformly ring-labeled {sup 14}C-TNT in a respirometer that trapped the evolved CO{sub 2}. Biodegradation behavior was characterized based on oxygen consumption, distribution of {sup 14}C activity, and high-performance liquid chromatography (HPLC) analysis of TNT and its transformation products.
- Sponsoring Organization:
- Department of the Army, Washington, DC (United States)
- OSTI ID:
- 490983
- Report Number(s):
- CONF-950483--; ISBN 1-57477-008-X
- Country of Publication:
- United States
- Language:
- English
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