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Title: Aerobic biotransformation and mineralization of 2,4,6-trinitrotoluene

Abstract

Respirometric mineralization studies of 2,4,6-trinitrotoluene (TNT) were conducted with microorganisms isolated from a site contaminated with munitions waste in Illinois. Nine aerobic bacterial species were isolated under a carbon- and nitrogen-limited condition and tentatively identified as: one Pseudomonas species; one Enterobacter species; and seven Alcaligenes species. Experiments were performed using each of the nine organisms individually and with a consortium of all nine bacterial species. The aerobic microorganisms were cultured in a sterile nutrient solution with glucose and 20 mg/L TNT. Mineralization was determined using uniformly ring-labeled {sup 14}C-TNT in a respirometer that trapped the evolved CO{sub 2}. Biodegradation behavior was characterized based on oxygen consumption, distribution of {sup 14}C activity, and high-performance liquid chromatography (HPLC) analysis of TNT and its transformation products.

Authors:
; ;  [1]
  1. Texas A and M Univ., College Station, TX (United States). Dept. of Civil Engineering
Publication Date:
Sponsoring Org.:
Department of the Army, Washington, DC (United States)
OSTI Identifier:
490983
Report Number(s):
CONF-950483-
ISBN 1-57477-008-X; TRN: IM9729%%234
Resource Type:
Conference
Resource Relation:
Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Bioremediation of recalcitrant organics; Hinchee, R.E.; Anderson, D.B. [eds.] [Battelle Memorial Inst., Columbus, OH (United States)]; Hoeppel, R.E. [ed.] [Naval Facilities Engineering Services Center, Port Hueneme, CA (United States)]; PB: 378 p.; Bioremediation, Volume 3(7)
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE; BIODEGRADATION; TNT; AEROBIC CONDITIONS; SITE CHARACTERIZATION; ILLINOIS; REMEDIAL ACTION; LIQUID COLUMN CHROMATOGRAPHY

Citation Formats

Bae, B.H., Autenrieth, R.L., and Bonner, J.S. Aerobic biotransformation and mineralization of 2,4,6-trinitrotoluene. United States: N. p., 1995. Web.
Bae, B.H., Autenrieth, R.L., & Bonner, J.S. Aerobic biotransformation and mineralization of 2,4,6-trinitrotoluene. United States.
Bae, B.H., Autenrieth, R.L., and Bonner, J.S. Sun . "Aerobic biotransformation and mineralization of 2,4,6-trinitrotoluene". United States. doi:.
@article{osti_490983,
title = {Aerobic biotransformation and mineralization of 2,4,6-trinitrotoluene},
author = {Bae, B.H. and Autenrieth, R.L. and Bonner, J.S.},
abstractNote = {Respirometric mineralization studies of 2,4,6-trinitrotoluene (TNT) were conducted with microorganisms isolated from a site contaminated with munitions waste in Illinois. Nine aerobic bacterial species were isolated under a carbon- and nitrogen-limited condition and tentatively identified as: one Pseudomonas species; one Enterobacter species; and seven Alcaligenes species. Experiments were performed using each of the nine organisms individually and with a consortium of all nine bacterial species. The aerobic microorganisms were cultured in a sterile nutrient solution with glucose and 20 mg/L TNT. Mineralization was determined using uniformly ring-labeled {sup 14}C-TNT in a respirometer that trapped the evolved CO{sub 2}. Biodegradation behavior was characterized based on oxygen consumption, distribution of {sup 14}C activity, and high-performance liquid chromatography (HPLC) analysis of TNT and its transformation products.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Dec 31 00:00:00 EST 1995},
month = {Sun Dec 31 00:00:00 EST 1995}
}

Conference:
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