Aromatic hydrocarbon degradation in hydrogen peroxide- and nitrate-amended microcosms
- Earth Technology, Grand Rapids, MI (United States)
Fifty microcosms were constructed using aquifer materials from a former coal gasification site and divided into four groups: poisoned control, nutrient-free control, hydrogen peroxide-amended, and nitrate-amended microcosms. Each microcosm contained site soil and groundwater in a 1.2-L glass media bottle. When depleted, hydrogen peroxide and sodium nitrate were injected into the microcosms. Microcosms were periodically sacrificed for analysis of polycyclic aromatic hydrocarbons (PAHs); monocyclic aromatic hydrocarbons (benzene, toluene, ethylbenzene, and xylenes [BTEX]); total petroleum hydrocarbons (TPH); and heterotrophic plate counts (HPCs). BTEX and two- and three-ringed PAHs were degraded in microcosms receiving electron-acceptor additions compared to poisoned controls. Four-, five-, and six-ringed PAHs were not significantly degraded during this study. Except in poisoned controls, significant amounts of dissolved oxygen (DO) or nitrate were utilized, and microbial populations increased by 3 to 5 orders of magnitude compared to site soils used to assemble the microcosms (i.e., baseline samples).
- OSTI ID:
- 490969
- Report Number(s):
- CONF-950483-; ISBN 1-57477-008-X; TRN: 97:011818
- Resource Relation:
- Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Bioremediation of recalcitrant organics; Hinchee, R.E.; Anderson, D.B. [eds.] [Battelle Memorial Inst., Columbus, OH (United States)]; Hoeppel, R.E. [ed.] [Naval Facilities Engineering Services Center, Port Hueneme, CA (United States)]; PB: 378 p.; Bioremediation, Volume 3(7)
- Country of Publication:
- United States
- Language:
- English
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