Structural organization and chromosomal assignment of the mouse embryonic TEA domain-containing factor (ETF) gene
- Kumamoto Univ. School of Medicine (Japan)
- National Institute of Radiological Sciences, Chiba (Japan); and others
Embryonic TEA domain-containing factor (ETF) belongs to the family of proteins structurally related to transcriptional enhancer factor-1 (TEF-1) and is implicated in neural development. Isolation and characterization of the cosmid clones encoding the mouse ETF gene (Etdf) revealed that Etdf spans approximately 17.9 kb and consists of 12 exons. The exon-intron structure of Etdf closely resembles that of the Drosophila scalloped gene, indicating that these genes may have evolved from a common ancestor. Then multiple transcription initiation sites revealed by S1 protection and primer extension analyses are consistent with the absence of the canonical TATA and CAAT boxes in the 5{prime}-flanking region, which contains many potential regulatory sequences, such as the E-box, N-box, Sp1 element, GATA-1 element, TAATGARAT element, and B2 short interspersed element (SINE) as well as several direct and inverted repeat sequences. The Etdf locus was assigned to the proximal region of mouse chromosome 7 using fluorescence in situ hybridization and linkage mapping analyses. These results provide the molecular basis for studying the regulation, in vivo function, and evolution of Etdf. 29 refs., 5 figs., 1 tab.
- OSTI ID:
- 486311
- Journal Information:
- Genomics, Vol. 36, Issue 2; Other Information: PBD: 1 Sep 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
GENES
DNA-CLONING
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA SEQUENCING
GENETIC MAPPING
BIOLOGICAL EVOLUTION
GENE REGULATION
TRANSCRIPTION
SPLICING
CHROMOSOMES
TRANSCRIPTION FACTORS
DROSOPHILA
MICE
EXONS
INTRONS
COSMIDS
DNA HYBRIDIZATION
FLUORESCENCE
NUCLEOTIDES
BIOLOGICAL MARKERS