STUDIES OF NUCLEIC ACID AND PROTEIN SYNTHESIS IN ESCHERICHIA COLI. Progress Report
Studies, using tracer techniques, were carried out to clarify previous results with E. coli K-12 showing that protein synthesis can occur at very low levels of nucleic acid synthesis. The status of the mandatory coupling hypothesis, evaluating protein and nucleic acid synthesis in terms of requirements for net formation vs turnover and the nature of the control exerted by ribose and ribose precursors, is discussed. When nucleic acid synthesis was reduced by 95% or more by omission of ribose precursors from the medium, no net synthesis of protein could be detected by the Folin-Cicolteau technique. An investigation of deficiencies in the system that would prevent net synthesis from occurring was carried out. Studies with labeled compounds indicated that protein synthesis, resulting from turnover of existing protein, can occur when nucleic acid synthesis is at least 95% inhibited, but that net protein synthesis may not occur in the absence of net nucleic acid synthesis. (M.C.G.)
- Research Organization:
- Albany Medical Coll., N.Y.
- NSA Number:
- NSA-15-027369
- OSTI ID:
- 4843789
- Report Number(s):
- TID-13100
- Country of Publication:
- United States
- Language:
- English
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