LPP, the preferred fusion partner gene of HMGIC in lipomas, is a novel member of the LIM protein gene family
- Univ. of Leuven and Flanders Interuniversity Inst. for Biotechnology (Belgium); and others
A major cytogenetic subgroup of lipomas is characterized by recurrent chromosome aberrations, mainly translocations, that involve chromosome segment 12q13-q15. Multiple chromosomes have been found as the translocation partners of chromosome 12 but 3q27-q28 is preferentially involved. In previous studies, it has been shown that the high mobility group (HMG) protein gene HMGIC at 12q15 is consistently rearranged as a consequence of these translocations. Here, we report the identification and characterization of the chromosome 3-derived translocation partner gene, which we have designated LPP (lipoma preferred partner gene). The gene was found to span a genomic region of over 400 kb. Nucleotide sequence analysis of a composite cDNA of LPP revealed an open reading frame of 1836 nucleotides encoding a proline-rich protein containing a leucine-zipper motif in its amino-terminal region and three LIM domains in its carboxy-terminal region. The LPP-encoded protein should be classified as a novel member of the group 3 proteins of the LIM protein gene family. Using reverse transcriptase combined with polymerase chain reactions in the analysis of a number of lipoma cell lines and primary lipomas, it appeared that LPP is frequently rearranged also in cases without a cytogenetically detectable involvement of 3q27-q28. 39 refs., 6 figs., 1 tab.
- OSTI ID:
- 484332
- Journal Information:
- Genomics, Vol. 36, Issue 1; Other Information: PBD: 15 Aug 1996
- Country of Publication:
- United States
- Language:
- English
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BASIC STUDIES
GENES
GENETIC MAPPING
SPLICING
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA SEQUENCING
TISSUE DISTRIBUTION
GENE REGULATION
DNA-CLONING
TRANSCRIPTION
HUMAN CHROMOSOME 12
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
HUMAN CHROMOSOME 3
MAN
NEOPLASMS
HEREDITARY DISEASES
PROTEINS
GENE AMPLIFICATION
DNA HYBRIDIZATION
FLUORESCENCE
CONTIGS
NUCLEOTIDES
POLYMERASE CHAIN REACTION