CARBON-14 FIXATION IN POLLEN OF YELLOW LUPINE (LUPINUS LUTEUS LINN.)
Carbon-14 fixation studies were made on germinated pollen of yellow lupine to ascertain whether the chlorophyll reported to be in these grains was functional photosynthetically. Light and dark exposures to atmospheres containing 20 and 500 mu c of carbon-14 labeled carbon dioxide were made for 1.5 and 45 minutes, respectively. The exposed pollen was extracted in 80% ethanol, the resulting extract reduced in volume, and chromatographed two dimensionally. When the chromatograms were cut inio numbered small squares and their activity counted in an automatic sample counting system, a marked similarity was observed in the pattern of radioactivity from all exposures. Eluting and co- chromatographing this activity from the squares, with known standards, demonstrated labeling to be specific to certain intermediates of the Krebs cycle and their derived amine acids. The labeling in these intermediates and the absence of labeling in photosynthetic metabolites is strong evidence that only respiratory fixation of carbon-14 occurs in the germinated pollen of this variety of yellow lupine under the conditions of the experiment. (auth)
- Research Organization:
- Kansas. Agricultural Experiment Station, Manhattan
- DOE Contract Number:
- AT(11-1)-1015
- NSA Number:
- NSA-16-021890
- OSTI ID:
- 4838138
- Report Number(s):
- TID-15982
- Resource Relation:
- Other Information: Orig. Receipt Date: 31-DEC-62
- Country of Publication:
- United States
- Language:
- English
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