Determination of Excitation Spectra with a Recording Spectrophotometer

Journal Article · Sun Oct 01 04:00:00 EDT 1961 · Analytical Chemistry

DOI:https://doi.org/10.1021/ac60179a037· OSTI ID:4837748

Sill, C. W.

A simple and inexpensive accessory was developed to permit the determination of excitation spectra of fluorescent materials with a recording spectrophotometer. The accessory consists of three front-surfaced mirrors arranged so that the light from the monochromator is redirected to pass through a sample cell at a right angle to the direction taken by the fluorescent light from the cell to the phototube compartment. The signal from the detector reflects the change in intensity of fluorescent light as a function of the wave stray light characteristics of the resulting spectra are as good as those of the spectrophotometer used. After correcting for the emission characteristics of the light source, excitation spectra of fluorescent materials parallel closely their absorption spectra and can be used in a similar manner as an analytical tool. However, the excitation spectra may be thousands of times more sensitive and applicable in the presence of other absorbing but nonfluorescent species that would interfere seriously with absorption measurements. Several examples of practical importance are given.

• Research Organization: U.S. Atomic Energy Commission, Health and Safety Div., Idaho Falls, Idaho
• Sponsoring Organization: USDOE
• NSA Number: NSA-15-030863
• OSTI ID: 4837748
• Journal Information: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 11 Vol. 33; ISSN 0003-2700
• Publisher: American Chemical Society (ACS)
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ABSORPTION
ENGINEERING AND EQUIPMENT
FLUORESCENCE
FREQUENCY
MONOCHROMATORS
PHOTOELECTRIC CELLS
QUANTITATIVE ANALYSIS
RECORDING SYSTEMS
SAMPLING
SENSITIVITY
SIGNALS
SPECTROMETERS