Effects of in Vitro X-Irradiation on the Survival of Mouse Eggs
A procedure for the in vitro x irradiation of unfertilized mouse eggs is described. Unfertilized oviducal eggs, obtained by superovulation of prepuberal C3H mice, were treated in vitro with doses of x irradiation rangingg from l0 to 900 r and transplanted to recipient Cal A females in which fertilization and developmeni occurred. Recipieni females were autopsied 17 to l9 days later. Living and dead fetuses from donor and native eggs were identified and counted, and resorption sites were counted. The number of transplanted eggs failing to implant or dying very shortly after implantation was calculated. Thirty-eight per cent of control nonirradiated eggs survived and developed to term; in general, per cent survival decreased with increasing doses of irradiation. Death occurred at several developmental stages after irradiation. Only l% of oscytes receiving lethal doses of irradiation survived longer than the twelfth day of gestation; a higher proportion apparently died after implantation but before eye color could be distinguished. Death prior to implantation showed the best correlation with amount of x ray administered; it increased sharply with increasing dosage of irradiation. The data are represented by the following dosage classification; LD/sub 10-20/ 10 to 100 r; LD/sub 40-50/ 200 to 300 r; LD/ sub 700 r; LD s/ub 99-99.9 750 to 900 r.
- Research Organization:
- Univ. of California, San Francisco
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-16-017524
- OSTI ID:
- 4835509
- Journal Information:
- Radiation Research, Vol. 16, Issue 5; Other Information: Orig. Receipt Date: 31-DEC-62; ISSN 0033-7587
- Publisher:
- Radiation Research Society
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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