Effect of ultraviolet inactivation and photoreactivation on the induced synthesis of β-galactosidase by Escherichia coli
Journal Article
·
· Archives of Biochemistry and Biophysics
The inactivation of the synthesis of induced BETA -galactosidase by cells of Escherichia coli by ultraviolet irradiation is described. Synthesis of enzyme can be preferentially restored by photoreactivation. Examination of crude cell extracts by zone electrophoresis revealed the presence of a particle-bound enzyme that did not migrate in the electric field. The particle-bound enzyme is shown to exist only as long as inducer is present. The significance of these observations with respect to the possible mechanism of enzyme synthesis is discussed.
- Research Organization:
- Oak Ridge National Lab., Tenn.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-16-021923
- OSTI ID:
- 4827748
- Journal Information:
- Archives of Biochemistry and Biophysics, Vol. 97, Issue 3; Other Information: Orig. Receipt Date: 31-DEC-62; ISSN 0003-9861
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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