Recovery from premutational damage of X irradiation inDrosophila spermatogenesis

Journal Article · Fri Dec 01 04:00:00 EST 1961 · Journal of Cellular and Comparative Physiology

DOI:https://doi.org/10.1002/jcp.1030580420· OSTI ID:4819706

Sobels, F. H.; Tates, A. D.

Data relating to repair processes in Drosophila males are summarized. The data suggest that repair from pre-mutational damage is possible in at least three different stages of spermatogenesis, that is spermatids, meiotic stages, and late spermatogonia. Most data are available for stages characterized by peak sensitivity to x irradiation, presumably consisting of spermatids and spermatocytes. Here fractionation of the dose and pretreatment with chloramphenicol lower the mutagenic effects of radiation, whereas posttreatment with nitrogen results in an increase of radiation-mutagenicity. Posttreatment with cyanide may result in either an increase or a decrease of the mutation- frequency. As pointed out before, these observations can be explained by assuming that two processes with contrasting effects are involved in repair from premutational damage. The observation that pretreatment with chloramphenicol results in an increase of the radiosensitivity in sperm and tn a decrease of that in spermatids may help to understand the pronounced differences in radiosensitivity between these two stages. Recovery seems to occur in dividing cells as meiotic stages and late spermatogonia where the mutagentc effect of radiation is reduced by pretreatment with chloramphenicol and enhanced by anoxia, following radiation. The observation that in dividing germ cells of Droso~ila completion of the radiation-initiated mutation process is associated with protein synthesis is in good agreement with the findings in lower organisms like Paramecium and bacteria. Also, it may be pointed out that there is a fair correspondence between the course of spermatogenesis in Drosophila and in the mouse. In the mouse the work of Russell and coworkers gives a clear indication that repair of initial radiation damage in gonia is possible. In view of the present Drosophila data it is tempting to believe that there is a uniform pattern to which repair-processes conform in dividing cells of widely divergent organisms.

• Research Organization: State Univ. of Leiden
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-007454
• OSTI ID: 4819706
• Journal Information: Journal of Cellular and Comparative Physiology, Journal Name: Journal of Cellular and Comparative Physiology Journal Issue: S1 Vol. 58; ISSN 0095-9898
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
ANTIBIOTICS
BACTERIA
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CHLORAMPHENICOL
CYANIDES
GONADS
INSECTS
MEIOSIS
MICE
MICROORGANISMS
MITOSIS
MUTATIONS
NITROGEN
OXYGEN
PROTEINS
RADIATION DOSES
RADIATION INJURIES
RADIOSENSITIVITY
RECOVERY
X RADIATION