THE EFFECTS OF HORMONES AND X-IRRADIATION ON THE SURVIVAL OF ISOGRAFTED FETAL MOUSE HEARTS
Work concerned with the isografting of fetal mouse hearts and the effects of the recipient's sex, adrenocorticotrophic hormone, cortisone acetate topically and parenterally, and X-irradiation on the survival of these hearts is described. The fetal mouse hearts were obtained from mice of strain C3H/JAX in the final days of gestation. The hearts were removed using sterile technique and placed upon the exposed blood vessel under the (cellulose acetateSaran) chamber on the recipient's back. The hearts were then observed by microscopic examination for viability of the tissue. The cardiac tissue was considered viable as lcng as pulsatile activity could be observed. Survival time was considered as the time from the day of transplantation to the day of cessation of pulsation and was recorded in days. The mean survival time for fetal cardiac tissue isografted on untreated male mice was 30.00 days. Statistical comparison was made of means enwploying the F'' test and the t'' test with a probability level of 5%. The sex of the recipient animal had no effect on the survival of isografted fetal tissue as demonstrated by a P > 0.05 on statistical comparison of means between male and female animals. However, castrated males showed a significant decrease in survival time which was not apparent in castrated females. Whether the significant decrease in survival shown by castrated males can be attributed to the absence of testosterone needs further elucidation. The irradiation of the host animal with 250 r of x rays 24 hr prior to transplantation showed no significant increase in survival time. A dosage of 400 r proved fatal to all mice submitted to this exposure. Adrenocorticotropic hormone likewise demonstrated little effect on survival time in the dosage employed. The action of ACTH on the adrenal cortex caused the production of the glucocorticoids and mineralocorticoids. The mineralocorticoids were prophlogistic in nature and probably accounted for the rejection of the isografted heart tissue. Intramuscular injections of cortisone acetate gave the most profound results. The mean survival time was 49.l4 days and when statistically compared with untreated males a highly significant difference was revealed (P < 0.001). Histological studies of the graft bed show resorption occurring. lt was concluded from these data that by employing genetically-related donors and subsequent cortisone therapy more prolonged survival of transplanted tissue may be assured.
- Research Organization:
- Univ. of Nebraska, Lincoln
- NSA Number:
- NSA-16-021936
- OSTI ID:
- 4815304
- Journal Information:
- Dissertation Abstr., Vol. Vol: 22; Other Information: Orig. Receipt Date: 31-DEC-62
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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