Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly report, October 1--December 31, 1996

doi:10.2172/481472

Abstract

In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, the author plans to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. He also plans to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein.

Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly report, October 1, 1995--December 31, 1995

Mishra, N.C. December 1995

The ability of Neurospora to solubilize and bioconvert coal was investigated. The coal solubilizing activity (CSA) was fractionated to isolate the enzyme responsible for this activity. The enzyme was purified in order to obtain the amino acid sequence. From that sequence potential oligonucleotide probes were synthesized and used to screen genomic library of Neurospora. The gene so identified was isolated. CSA appears to be an phenol oxidase or is tyrosinase.
DOI: 10.2172/245631

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Large scale solubilization of coal and bioconversion to utilizable energy. Fifth quarterly technical progress report, October 1, 1994--December 31, 1994

Mishra, N.C. February 1995

In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, the authors plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. The authors also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungusmore » or purified fungal protein.« less
Large scale solubilization of coal and bioconversion to utilizable energy. Eleventh quarterly technical progress report, April 1, 1996--June 30, 1996

Mishra, N.C. October 1996

Neurospora has the capability to solubilize coal and the protein fraction accounting for this ability has been isolated. During this period the cola solubilizing activity (CSA) was fractionated and partially sequenced. The activity has been determined to be a tyrosinase and/or a phenol oxidase. The amino acid sequence of the protein was used to prepare oligonucleotides to identify the clone carrying Neurospora CSA. It is intended to clone the Neurospora gene into yeast, since yeast cannot solubilize coal, to further characterize the CSA.
DOI: 10.2172/419268

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Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly report, July 1, 1996--September 30 1996

Mishra, N.C. December 1996

A purification of the Neurospora protein with coal solubilization activity (CSA) using DEAE cellulose chromatography is described. The protein is heavily glycosylated suggesting that it is different than tyrosinase or common phenol oxidases even though it resembles these proteins in enzyme activity and molecular weight.
DOI: 10.2172/458598

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Large scale solubilization of coal and bioconversion to utilizable energy. Technical progress report, January 1--March 31, 1996

Mishra, N.C. May 1996

In order develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, the authors plan to clone the genes encoding Neurospora protein that facilitates depolymerization of coal. They also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the products of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purifiedmore »« less
DOI: 10.2172/650129

Full Text Available

Title: Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly report, October 1--December 31, 1996

Abstract

Citation Formats

Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly report, October 1, 1995--December 31, 1995

Large scale solubilization of coal and bioconversion to utilizable energy. Fifth quarterly technical progress report, October 1, 1994--December 31, 1994

Large scale solubilization of coal and bioconversion to utilizable energy. Eleventh quarterly technical progress report, April 1, 1996--June 30, 1996

Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly report, July 1, 1996--September 30 1996

Large scale solubilization of coal and bioconversion to utilizable energy. Technical progress report, January 1--March 31, 1996