RadioimmunoPET: Detection of colorectal carcinoma with positron-emitting copper-64-labeled monoclonal antibody

Journal Article · Sun Oct 01 00:00:00 EDT 1995 · Journal of Nuclear Medicine

OSTI ID:481205

Philpott, G W; Schwarz, S W; Anderson, C J ^[1]

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1. Washington Univ. School of Medicine, St. Louis, MO (United States)

Detection of tumor foci may be improved by combining the selective tumor-targeting properties of a monoclonal antibody with the superior sensitivity and contrast resolution of PET. An anti-colorectal carcinoma monoclonal antibody (MAb 1A3) was labeled with {sup 64}Cu, a positron-emitting radionuclide, by use of a bifunctional chelate (bromoacetamidobenzyl-TETA) and evaluated in 36 patients with suspected advanced primary or metastatic colorectal cancer. After radiopharmaceutical injection (5-20 mg protein, 10 mCi {sup 64}Cu), PET was performed once or twice, 4 to 36 hr later. All patients had CT scans and 18 patients were also studied with [{sup 18}F]fluorodeoxyglucose (FDG)PET. In 29 patients, one or more tumor sites (n= 56) were proven, in 5 patients the absence of active tumor was confirmed and in the remaining 2, tumor status is not yet confirmed. Of the 56 confirmed tumor sites, 40 were detected by MAb-PET as foci of increased activity (sensitivity 71%). The positive predictive value of MAb-PET was excellent, ranging from 89% (40/45) to 96% (43/45), depending on the ultimate classification of three image-positive, but as yet unconfirmed tumor sites. Also, MAb-PET detected 11 new occult tumor sites, including 9 small abdominopelvic foci less than 2.0 cm in diameter that were not detected by CT or MRI. There were no complications, but significantly elevated HAMA titers were found in 28% of the 29 patients tested 1 to 12 mo after injection. There was no apparent dose-related effect from 5 to 20 mg MAb 1A3. These Phase I/II results suggest that PET with radiolabeled MAbs (radioimmunoPET) may have important applications in clinical oncology, particulary for detecting smaller colorectal tumor foci in the abdomen or pelvis and for determining accurate dosimetry. 34 refs., 5 figs., 4 tabs.

• DOE Contract Number: FG02-87ER60512
• OSTI ID: 481205
• Journal Information: Journal of Nuclear Medicine, Vol. 36, Issue 10; Other Information: PBD: Oct 1995
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
55 BIOLOGY AND MEDICINE
BASIC STUDIES
CARCINOMAS
DETECTION
LARGE INTESTINE
RADIOIMMUNOSCINTIGRAPHY
EVALUATION
SENSITIVITY
RESOLUTION
QUALITY CONTROL
COPPER 64
RADIONUCLIDE KINETICS
RADIOPHARMACEUTICALS
DOSE-RESPONSE RELATIONSHIPS
DOSIMETRY
ACCURACY
MONOCLONAL ANTIBODIES
RADIOIMMUNOTHERAPY
CHELATES
PATIENTS
FLUORODEOXYGLUCOSE
IMAGES
EMISSION COMPUTED TOMOGRAPHY