Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells. II. Quantitative analysis of giant polynuclear cell formation
The determination of cell number, DNA content, and number of nuclei along the course of giant polynuclear cell formation in HVJ - ETC system led to the conclusion that giant polynuclear cell formation in this system is due to fusion of the tumor cells themselves and not to any kind of cell growth. The decrease in cell number during the fusion reaction indicates the degree of cell fusion. The grade of the fusion reaction can be quantitatively expressed by the fusion index: Fusion index = (Cell number in nonvirus control tube/Cell number in test tube) --1.0. The fusion reaction required the adsorption of many virus particles onto the cells, i.e. 1300 adsorbed virus particles per cell was required of 50% decrease of the cell number, and the grade of fusion increase with increasing number of adsorbed virus particles. The fusion activity of HVJ is a new character differing from other activities such as infectivity, hemagglutinating activity, hemolytic activity, and sialidase activity of the virus.
- Research Organization:
- Osaka Univ.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-16-012851
- OSTI ID:
- 4797315
- Journal Information:
- Exptl. Cell Research, Vol. Vol: 26; Other Information: Orig. Receipt Date: 31-DEC-62
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
Reprogramming of somatic cells induced by fusion of embryonic stem cells using hemagglutinating virus of Japan envelope (HVJ-E)
Hemagglutinating virus of Japan envelope (HVJ-E) can enhance the immune responses of swine immunized with killed PRRSV vaccine