Hydrolyzable hemoglobin adducts of polyfunctional monocyclic N-substituted arenes as dosimeters of exposure and markers of metabolism
- Universitaet Wuerzburg (Germany)
Hemoglobin adducts of 10 polyfunctional amino- and nitro-substituted benzenes and toluenes were analyzed: 2,4,6-trinitrotoluene, 2,4- and 2,6-dinitrotoluene. 2-amino-4-nitrotoluene, 4-amino-2-nitrotoluene, 2,4- and 2,6-diaminotoluene, 1,3-dinitrobenzene, 1-amino-3-nitrobenzene, and 1,3-diaminobenzene. A single dose (0.5 mmole/kg) of the test compounds was administered to female Wistar rats by gavage, and blood extracted and hemoglobin prepared after 24 hr. One or more cleavage products could be obtained in each case by hydrolyzing hemoglobin (Hb). Hemoglobin binding indices (HBl); binding (mmole/mole Hbl/dose [mmole/kg]) and the ratios of hydrolyzable adducts were determined. The HBl ranged between <0/.02 and 69.0. The results indicate the in vivo formation of several, convalently bound, hydrolyzable hemoglobin adducts. Conclusion on prevailing metabolic pathways can be drawn. Total binding of several compounds seems sufficient for biomonitoring of human blood samples. These chemicals are considered representative for environmental contamination with explosives of this type, and we propose their Hb adducts be used as dosimeters for human exposure to these suspected carcinogens. 8 refs., 2 figs., 2 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 478799
- Report Number(s):
- CONF-9210376--
- Journal Information:
- Environmental Health Perspectives, Journal Name: Environmental Health Perspectives Journal Issue: Suppl.6 Vol. 102; ISSN 0091-6765; ISSN EVHPAZ
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
54 ENVIRONMENTAL SCIENCES
55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ADDUCTS
BIOLOGICAL EFFECTS
CARCINOGENS
CHEMICAL EXPLOSIVES
COMBUSTION PRODUCTS
HEMOGLOBIN
METABOLISM
NITROBENZENE
POLLUTANTS
RATS
RISK ASSESSMENT
TNT