Nitroreduction and formation of hemoglobin adducts in rats with a human intestinal microflora
- Univ. of Nijmegen (Netherlands)
In the covalent binding of nitroarenes to macromolecules, nitroreduction is an important step. The intestinal microflora represents an enormous potential of bacterial nitroreductase activity. As a consequence, the in vivo nitroreduction of orally administerednitroarenes is primarily located in the intestine. In this study, we have investigated the nitroreduction of 2-nitrofluorene (2-NF) by a human microflora in female Wistar rats. Germ-free (FG) rats were equipped with a bacterial flora derived from human feces. Nontreated GF rats and GF animals equipped with a conventional rat flora were used as controls. The composition of the human and the conventional microflora isolated from the rats were consistent with the microflora of the administered feces. In the rats receiving only sunflower seed oil, no adducts were detected. The animals equipped with a human or rat microflora that received 2-aminofluorene (2-AF) formed 2-AF hemoglobin (Hb)-adducts at average levels mean {+-} 0.003 and 0.043 {+-} 0.010 {mu}mole/g Hb, respectively. In the FG rats, an adduct level of 0.57 {+-} 0.09 was determined after 2-AF administration and non adducts were detected after 2-NF administration. The results show that nitroreduction by an acquired human intestinal microflora and subsequent adduct formation can be studied in the rate in vivo. 21 refs., 3 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 478798
- Report Number(s):
- CONF-9210376-; ISSN 0091-6765; TRN: 97:002101-0004
- Journal Information:
- Environmental Health Perspectives, Vol. 102, Issue Suppl.6; Conference: 5. international conference on carcinogenic and mutagenic n-substituted aryl compounds, Wursburg (Germany), 18-21 Oct 1992; Other Information: PBD: Oct 1994
- Country of Publication:
- United States
- Language:
- English
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