Structure of the mouse Saa4 gene and its linkage to the serum amyloid A gene family
- Univ. of Kentucky Medical Center, Lexington, KY (United States); and others
The serum amyloid A (SAA) proteins are a polymorphic family of apolipoproteins associated with high-density lipoproteins (HDL). Three distinct subfamilies have been identified: (i) a cytokine-induced acute phase subfamily that is hepatically produced and can become the major apolipoprotein on HDL (SAA1, SAA2); (ii) a peripherally produced acute phase SAA3 that is only a monor HDL apolipoprotein; and (iii) a constitutive subfamily (SAA4) that is a minor normal HDL apolipoprotein comprising more than 90% of the SAA during homeostasis. Here we define the structure of the Saa4 gene. Similar to other Saa family members, it has four exons and three introns. It is 4588 bp long from the transcription start site to the end of the 3{prime}-untranslated region and is approximately 20% larger than other Saa genes. We have located Saa4 11 kb upstream from Saa3 and 5 kb downstream from Saa1, with the pseudogene approximately 1 kb from the 5{prime} end of Saa4. Saa4 has the same orientation as most other Saa family members, with only Saa2 having an opposing orientation. These data promote our understanding of the evolution of the Saa family. They enhance our ability to develop the mouse as a transgenic and gene deletion model to advance the understanding of the function of these apolipoproteins. 20 refs., 2 figs., 1 tab.
- OSTI ID:
- 478571
- Journal Information:
- Genomics, Vol. 34, Issue 1; Other Information: PBD: 15 May 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
TRANSGENIC MICE
BIOLOGICAL MODELS
APOLIPOPROTEINS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION
BIOLOGICAL EVOLUTION
GENE MUTATIONS
DNA-CLONING
DNA SEQUENCING
SIZE
GENE REGULATION
METABOLISM
POLYMERASE CHAIN REACTION
NUCLEOTIDES
AMINO ACID SEQUENCE
EXONS
INTRONS