QUANTITATIVE STUDIES ON THE RADIATION PATHOLOGY OF THE BONE MARROW OF SMALL LABORATORY MAMMALS. I. THE FILM-RATIO METHOD: ABSOLUTE COUNTS FROM FILMS PREPARED WITH SUSPENSIONS OF BONE MARROW CELLS
A film-ratio method was devised in which absolute counts of suspended bone marrow cells were acheived by means of a lasting film on a microscopic slide, prepared with a medium containing standard particles (spores) in a known concentration. The ratio of cells to spores was determined by microscopic examination of the film. The marrow cell count = R (cell-to-particle ratio in film) times N (number of standard particles in marrow suspension). Such films have the advantage of not requiring immediate counting. Their examination is no more laborious than the conventional method (chamber count plus differential count). Uniformity trials were carried out, sources of variance were explored, and results were compared with those obtained with the hemacytometer and with the electronic counter. The method was shown to be practical in a trial experiment dealing with changes in the marrow of irradiated mice. (auth)
- Research Organization:
- Univ. of California School of Medicine, San Francisco
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-16-032798
- OSTI ID:
- 4780297
- Journal Information:
- Radiation Res., Vol. Vol: 17; Other Information: Orig. Receipt Date: 31-DEC-62
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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