In Vivo and in Vitro Recovery of Irradiated Gametes of Arbacia punctulata

Journal Article · Thu Nov 01 04:00:00 EST 1962 · Radiation Research

DOI:https://doi.org/10.2307/3571224· OSTI ID:4776126

Failla, Patricia McClement

The recovery from radioinduced cleavage delay in Arbacia eggs left inside the irradiated urchin for various lengths of time after irradiation (in vivo recovery) was investigated. The rate of recovery was less by a factor of nearly 100 than when irradiated eggs were left in sea water for a time before fertilization (in vitro recovery). The irradiation of eggs and sperm in vivo caused a cleavage delay about 20% less than that induced in the same gametes irradiated in vitro. A decrease in effect of about the same magnitude was observed when the eggs and sperm were irradiated in vitro in the absence of oxygen. That the lower in vivo recovery rate is not caused by a lower oxygen tension in vivo, however, was indicated by the complete independence of the in vitro recovery rate on the presence of oxygen. It was also found that the eggs were no longer capable of recovering rapidly in vitro after they had remained inside the irradiated animal for 4 or more hours. (auth)

• Research Organization: Argonne National Lab., Ill.
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-001324
• OSTI ID: 4776126
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 5 Vol. 17; ISSN 0033-7587
• Publisher: Radiation Research Society
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
ANIMALS
ARBACIA
BIOLOGY AND MEDICINE
EGGS
GONADS
IN VITRO
IN VIVO
OXYGEN
RADIATION INJURIES
RECOVERY
REPRODUCTION
SEA
SPERM
SUSPENSIONS