"MUTATIONAL SYNERGISM" OF ULTRAVIOLET LIGHT AND CAFFEINE IN ESCHERICHIA COLI

Journal Article · Sat Sep 01 04:00:00 EDT 1962 · Journal of Bacteriology (U.S.)

OSTI ID:4773337

Shankel, D M

When ultraviolet-induced mutations of Escherichia coli B/r develop in the presence of caffeine, there is a large increase in mutant numbers. The development of these extra mutants is not affected by the presence of normally occurring purines and pyrimidines, azauracil, azathymine, or 5-methyltryptophan, but many of the mutants can be photoreversed. They are stabilized or lost during the first hour of postirradiation growth. Enzymntically active materials extracted from E. coli cells do not interfere with the caffeine effect, and variation of the growth temperature during the development of the mutations fails to alter the effect. Prefeeding the cells with caffeine prior to irradiation does not produce the response. The effect is observable with both stationary- phase and log-phase populations, but is not observable when a relatively high dosage of ultraviolet light is employed. (auth)

• Research Organization: Univ. of Kansas, Lawrence
• NSA Number: NSA-17-001294
• OSTI ID: 4773337
• Journal Information: Journal of Bacteriology (U.S.), Journal Name: Journal of Bacteriology (U.S.) Vol. Vol: 84; ISSN JOBAA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ALKALOIDS
AMINO ACIDS
BACTERIA
BIOLOGY AND MEDICINE
CAFFEINE
ENZYMES
ESCHERICHIA COLI
METABOLISM
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMISTRY
POPULATIONS
PURINES
PYRIMIDINES
RADIATION DOSES
RADIATION EFFECTS
TEMPERATURE
ULTRAVIOLET RADIATION
VARIATIONS