Chromosomal localization of the human gene encoding c-myc promoter-binding protein (MPB1) to chromosome 1p35-pter

Journal Article · Sat Feb 01 04:00:00 EST 1997 · Genomics

DOI:https://doi.org/10.1006/geno.1996.4499· OSTI ID:476894

White, R A; Dowler, L L ^[1]; Adkison, L R ^[2]; Ray, R B ^[3]

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1. Univ. of Missouri, Kansas City, MO (United States)
2. Mercer Univ. School of Medicine, Macon, GA (United States)
3. St. Louis Univ. Health Sciences Center, St. Louis, MO (United States)

We report the mapping of the human gene MPB1 (c-myc promoter binding protein), a recently identified gene regulatory protein. MPB1 binds to the c-myc P2 promoter and exerts a negative regulatory role on c-myc transcription. Since exogenous expression from transfection of the MPB1 gene suppresses the tumorigenic property of breast cancer cells, there was interest in determining the chromosomal location of this gene. The human MPB1 gene was assigned to human chromosome 1p35-pter using Southern blot analyses of genomic DNAs from rodent-human somatic hybrid cell lines. A specific human genomic fragment was observed only in the somatic cell lines containing human chromosome 1 or the p35-pter region of the chromosome. 10 refs., 2 figs.

• OSTI ID: 476894
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 39; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CHROMOSOMAL ABERRATIONS
DNA HYBRIDIZATION
GENE REGULATION
GENE REPRESSORS
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 1
HUMAN CHROMOSOME 17
HUMAN CHROMOSOME 21
HYBRIDIZATION
MAMMARY GLANDS
PROTEINS
SOMATIC CELLS
TRANSCRIPTION
TUMOR CELLS