Construction and characterization of a human bacterial artificial chromosome library
- California Institute of Technology, Pasadena, CA (United States); and others
We have constructed an arrayed human genomic BAC library with approximately 4X coverage that is represented by 96,000 BAC clones with average insert size of nearly 140 kb. A new BAC vector that allows color-based positive screening to identify transformants with inserts has increased BAC cloning efficiency. The library was gridded onto hybridization filters at high density for efficient identification of BAC clones by colony hybridization. The library was also formulated into characteristic DNA pools to allow for PCR screening of the library mainly by screening with more than 300 different landmarks that include cDNA, STSs, and cosmid clones. We describe methods for using BAC clones and discuss the implications for genome characterization, mapping, and sequencing. 25 refs., 5 figs., 1 tab.
- DOE Contract Number:
- FG03-89ER60891
- OSTI ID:
- 476777
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 34; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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