ON OXIDATIVE METABOLISM IN STAPHYLOCOCCUS AUREUS AND A MUTANT OBTAINED BY ULTRAVIOLET IRRADIATION
The oxidative metabolism of Staphylococcus aureus-209 (St-209) and its mutant Staphylococcus aureus-UV-3 (StUV-3) was studied. The St-209 cells exhibited a drastic increase of oxygen uptake in the presence of glucose and oxidized also pyruvate, lactate, acetaldehyde, formalde hyde, ethanol, formic acid, oxalacetic acid, and malate. Under anaerobic conditions St-209 caused intensive decarboxylation of pyruvic and oxalacetic acid. Low concentrations of monoiodoacetate (0.2 mmol) completely inhibited glucose consumption and lactate accumulation by St-209. St-UV-3 did not show any increase in oxygen consumption in the presence of glucose but oxidized pyruvate, acetate, acetaldehyde, ethanol, oxalacetic acid, cis -aconitate, f umarate, succinate, and glyoxylate. Irregular malate and alpha -ketoglutarate oxidation was also noted. Under anaerobic conditions St-UV-3 only slightly decarboxylated pyruvic and oxalacetic acid. The Q/sub CO/sub 2//Qc/sub O/sub 2/ coefficient was more than 2 for pyrvate oxidation by St-209 while with St-UV-3 it approached 1. The effect was followed up of arsenite, malonate, cyanlde, and inorganic phosphate upon oxidation of some substrates. A discussion is presented of the pathways of oxidative transformations of respiratory substrates in St-209 and St-UV-3 as well as of the equivalency between the mutant and tumor tissue metabolism and of the possible mechanism of the effect of cancerostatic compounds upon St-UV-3 cells. (auth)
- Research Organization:
- Tarasevich State Control Inst. for Medical and Biologic Preparation, USSR
- NSA Number:
- NSA-17-005790
- OSTI ID:
- 4755178
- Journal Information:
- Biochemistry (USSR) (English Transl.), Journal Name: Biochemistry (USSR) (English Transl.) Vol. Vol: 27
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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