THE INFLUENCE OF X RAYS ON ORGANELLE INDUCTION AND DIFFERENTIATION IN GRASSHOPPER SPERMATOGENESIS
The effects or x radiation on grasshopper spermatogenesis were studied by light and electron microscopy. The insects were irradiated at the second instar prior to the presence of maturation stages and observed at the last instar and imago stages. Dosages of 100 to 800 r retarded the differentiation of the nucleus and mitochondrial nebenkern in spermatids. Irradiation caused a curtailment and disorganization in the differentiation of the nebenkern from mitochondria. It also induced the formation of supernumerary centrioles, flagellar filaments, and acrosomes; nuclear disorganization as well as pycnosis and fragmentation also occurred. The nucleus appeared to be drawn toward each radioinduced supernumerary acrosome, with consequent multipolarity of the nucleus. Induction of a set of flagellar filaments was seen only where the centriolar structure was in contact with the nucleus. Details are given of an organelle that is composed of anastomosed and interwoven cytoplasmic strands. The results show that radioinduced changes in cell morphology are preceded by either alteration or curtailment of organelle differentiation. Furthermore, organelle differentiation during spermatogenesis was altered by doses that did not visibly affect the antecedent mitotic, maturation, or meiotic divisions; grasshoppers irradiated during the third instar showed the first effects of radiation at the last instar. (P.C.H.)
- Research Organization:
- Argonne National Lab., Ill.
- NSA Number:
- NSA-17-014040
- OSTI ID:
- 4749914
- Journal Information:
- J. Biophys. Biochem. Cytol., Vol. Vol: 9; Other Information: Orig. Receipt Date: 31-DEC-63
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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