A 7-d toxicity test for marine pollutants using the Pacific mysid Mysidopsis intii. 1: Culture and protocol development
Journal Article
·
· Environmental Toxicology and Chemistry
- Oregon State Univ., Newport, OR (United States)
- Virginia Inst. of Marine Science, Gloucester Point, VA (United States). School of Marine Science
- The Scientist, Philadelphia, PA (United States)
- Academy of Natural Sciences, Philadelphia, PA (United States). Patrick Center for Environmental Research
- Environmental Protection Agency, Newport, OR (United States)
The purpose of this study was to develop a short term (7-d) toxicity test for marine pollutants that is practical to perform at sites remote from a source of test organisms. Laboratory culture methods were developed that allowed successful long-term rearing of populations of the mysid Mysidopsis intii, a species indigenous to the Pacific coast of the United States. Mysidopsis intii was found to have a short life cycle of 20 d at 20 C, making it useful for chronic toxicity tests. A 7-d toxicity test was developed by comparing the sensitivities of various life stages of M. intii to sodium dodecyl sulfate (SDS). The most sensitive end point was found to be growth (final body length) of juveniles during the first 7 d after release. The sensitivity of mysids to SDS decreased with increasing age. To allow for shipping newly released juveniles to remote sites, a 7-d test was evaluated starting with 2-d-old mysids. The median lethal concentration for M. intii exposed to SDS at ages from 2 to 9 d was 4.59 mg/L (95% confidence limits, 4.21--5.00), and the maximum acceptable SDS concentration was 4.99 mg/L for both survival and growth.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 474301
- Journal Information:
- Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 10 Vol. 15; ISSN 0730-7268; ISSN ETOCDK
- Country of Publication:
- United States
- Language:
- English
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