Cometabolic degradation of chlorinated solvents: Bacterial inhibition, inactivation, and recovery
- Oregon State Univ., Corvallis, OR (United States). Dept. of Civil Engineering
This paper summarizes an approach for quantifying degradation kinetics and bacterial activity changes during cometabolic degradation of chlorinated solvents, results from trichloroethylene (TCE) degradation experiments, and a mathematical model addressing fluctuations in activity caused by enzyme inhibition, inactivation, and respondent enzyme synthesis. Using Nitrosomonas duropaea as a slow-growing exemplar capable of effecting cometabolic transformations, quasi-steady-state ammonia oxidation was established in a small bioreactor. A chlorinated solvent was injected to perturb the system, and bacterial activity and solvent degradation were monitored. At TCE concentrations to about 3.5 mg/L, from slight to nearly complete ammonia monooxygenase (AMO) inactivation occurred without causing immediate cell death. Results suggested cellular injury was limited primarily to AMO, most metabolic systems remained functional, and bacterial recovery processes, independent of cell growth, were initiated while degrading TCE.
- OSTI ID:
- 474276
- Report Number(s):
- CONF-950483--; ISBN 1-57477-005-5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Factors limiting aliphatic chlorocarbon degradation by Nitrosomonas europaea: Cometabolic inactivation of ammonia monooxygenase and substrate specificity
A cometabolic kinetics model incorporating enzyme inhibition, inactivation, and recovery. 1: Model development, analysis, and testing