Accelerated biotransformation of carbon tetrachloride and chloroform by sulfate-reducing enrichment cultures
- Univ. of Illinois, Urbana, IL (United States). Dept. of Civil Engineering
- HDR Engineering Inc., Lake Oswego, OR (United States)
- Army Corps of Engineers, Champaign, IL (United States). Construction Engineering Research Labs.
The biotransformation of carbon tetrachloride (CT) and chloroform (CF) was examined with lactate- and acetate-grown sulfate-reducing enrichment cultures. Both cultures transformed CT, with approximately 50% reductively dechlorinated to CF and up to 10% to dichloromethane (DCM). Addition of cyanocobalamin increased the rate of CT transformation more than 100-fold. The principal product from [{sup 14}C]CT with cyanocobalamin added was carbon disulfide (CS{sub 2}); less than 3% was reduced to CF plus DCM. Autoclaved cultures that received cyanocobalamin were only one third as fast as their live counterparts, but produced similar amounts of CS{sub 2}. With CF, addition of cyanocobalamin to acetate- and lactate-grown cultures also increased the rate of transformation more than 100-fold. DCM was the principal transformation product until CF additions reached 270 mg/L, at which point almost no increase in DCM was observed. Thus, low levels of cyanocobalamin substantially accelerated the rate of CT and CF transformation and altered the distribution of products formed.
- Sponsoring Organization:
- Illinois State Government, Springfield, IL (United States)
- OSTI ID:
- 474270
- Report Number(s):
- CONF-950483-; ISBN 1-57477-005-5; TRN: IM9723%%160
- Resource Relation:
- Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Bioremediation of chlorinated solvents; Hinchee, R.E.; Leeson, A. [eds.] [Battelle Memorial Inst., Columbus, OH (United States)]; Semprini, L. [ed.] [Oregon State Univ., Corvallis, OR (United States)]; PB: 348 p.; Bioremediation, Volume 3(4)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Individual biotransformation rates in chlorinated aliphatic mixtures
Involvement of cytochromes in the anaerobic biotransformation of tetrachloromethane by Shewanella putrefaciens 200