The effects of ultraviolet-irradiated polyuridylic acid in cell-free protein synthesis in e. coli

Journal Article · Sat Sep 01 00:00:00 EDT 1962 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.48.9.1609· OSTI ID:4736199

Grossman, L.

The incorporation of U-C¹⁴-L-phenylalanine prepared from poiy-L- phenylaianine from Escherichia coli into acidinsoluble material and poly-L- phenylaianine following ultraviolet irradiation of poiyuridylic acid was examined. Irradiation of the polyuridylic acid with doses of ultraviolet light from 24 to 72 x 10⁴ ergs/mm² resulted in a marked depression in C¹⁴- phenyiaianine incorporation and was accompanied by an increase in C¹⁴- serine uptake into both acid-soluble material as well as polyphenylaianine.

• Research Organization: Brandeis Univ., Waltham, MA (United States)
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-007864
• OSTI ID: 4736199
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America, Vol. 48, Issue 9; Other Information: Orig. Receipt Date: 31-DEC-63; ISSN 0027-8424
• Publisher: National Academy of Sciences, Washington, DC (United States)
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
BACTERIA
BIOCHEMISTRY
CARBON 14
ESCHERICHIA COLI
ORGANIC ACIDS
PHENYLALANINE
PROTEINS
RADIATION DOSES
RADIATION EFFECTS
TRACER TECHNIQUES
ULTRAVIOLET RADIATION