A FLOW-THROUGH METHOD FOR SCINTILLATION COUNTING OF CARBON-14 AND TRITIUM IN GAS-LIQUID CHROMATOGRAPHIC EFFLUENTS

Journal Article · Sun Jul 01 04:00:00 EDT 1962 · Journal of Lipid Research (U.S.)

OSTI ID:4730547

Karmen, A; McCaffrey, I; Bowman, R L

A method for radioassay of gas-liquid chromatographic effluents by scintiliation counting is described. A cartridge filled with anthracene crystals is used as a flow-through radiation detector. Upon leaving the column, the effluent is passed over hot copper oxide, which converts the organic materials to CO/sub 2/ and water. If tritium is to be counted, the water is reacted with hot iron to release H and tritium gases. The effluent is then passed through the anthracene cartridge. The counting rate of the cartridge is monitored continuously during the analysis by a highly efficient, lowbackground scintiliation counter to yield a record of eluted radioactivity that resembles the conventional mass detector record. The detector is simply constructed, easily maintained, and insensitive to changes in gas composition but highly sensitive to radiation. High efficiency (more than 70% for C/sup 14/ and 20% for tritium) is coupled with low background counting rates ( approximates 15 cpm at C/ sup 14/ settings, 50 cpm at tritium settings). The counting rate returns to the low background rate following the detection of a labeled compound. (H.H.D.)

• Research Organization: National Heart Inst., Bethesda, Md.
• NSA Number: NSA-17-011027
• OSTI ID: 4730547
• Journal Information: Journal of Lipid Research (U.S.), Journal Name: Journal of Lipid Research (U.S.) Vol. Vol: 3; ISSN JLPRA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANTHRACENE
BACKGROUND
CARBON 14
CARBON DIOXIDE
CHROMATOGRAPHY
COPPER OXIDES
CRYSTALS
GAS FLOW
HYDROGEN
INSTRUMENTS AND INSTRUMENTATION
MONITORING
RADIATION DETECTORS
RADIOACTIVITY
SCINTILLATION COUNTERS
SENSITIVITY
TRACER TECHNIQUES
TRITIUM