Comparative radiosensitivity of forelimb and hindlimb primordia in Amblystoma punctatum
Journal Article
·
· Journal of Experimental Zoology
Embryos at stage 34, the earliest at which hindlimb rudiments can be located, were irradiated with 240-kv x rays at a rate of 152 r/min. The exposure dose was 1000 r and the absorbed dose estimated to be 950 rad. Within 10 to 90 min after irradiation, forelimb and hindlimb primordia were transplanted orthotopically to nonirradiated embryos of the same age. Both the irradiated and nonirradiated control grafts were examined daily for a period of 40 days. Development of the irradiated forelimb grafts was normal through stage 39. From this time until well past stage 46 all of the grafts were retarded in their differentiation and a large number of them became abnormally differentiated as early as stage 45. Development of the irradiated hindlimb grafts was normal in almost 1/4 of the cases from the earliest appearance of the limb bud. About 3/4 were either retarded or not yet formed during the earliest stages of development. At the same embryonic age the more mature forelimb rudiment is more radiosensitive than is the less mature hindlimb rudiment. The results suggest that radiosensitivity of an embryonic structure is not necessarily a function of chronological age but of respective levels of differentiation. (TCO)
- Research Organization:
- Univ. of Pennsylvania, Philadelphia
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-019851
- OSTI ID:
- 4725687
- Journal Information:
- Journal of Experimental Zoology, Journal Name: Journal of Experimental Zoology Journal Issue: 2 Vol. 150; ISSN 0022-104X
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
THE EFFECT OF TEMPERATURE UPON THE DEVELOPMENT OF X-IRRADIATED EAR GRAFTS IN AMBLYSTOMA
Restoration of regenerative ability of x-rayed newt limbs after grafting of proximal or distal skin
Interstrain variation in gamma-ray induction of hindlimb deformities in chick embryos
Journal Article
·
Wed Jan 31 23:00:00 EST 1962
· J. Exptl. Zool.
·
OSTI ID:4654282
Restoration of regenerative ability of x-rayed newt limbs after grafting of proximal or distal skin
Journal Article
·
Fri Sep 01 00:00:00 EDT 1978
· Growth; (United States)
·
OSTI ID:6540576
Interstrain variation in gamma-ray induction of hindlimb deformities in chick embryos
Journal Article
·
Fri Feb 01 00:00:00 EDT 1974
· Radiat. Res., v. 57, no. 2, pp. 332-341
·
OSTI ID:4307129