Variations in several responses of HeLa cells to x-irradiation during the division cycle

Journal Article · Mon Dec 31 23:00:00 EST 1962 · Biophysical Journal (U.S.)

DOI:https://doi.org/10.1016/S0006-3495(63)86801-0· OSTI ID:4719026

Terasima, T; Tolmach, L J

Several responses of synchronized populations of HeLa S₃ cells were measured after irradiation with 220 kev x rays at selected times during the division cycle. Survival as demonstrated by colony-forming ability, was maximal when cells were irradiated in the early post-mitotic (G₁) and the pre- mitotic (G₂) phases of the cycle, and minimal in the mitotic (M) and late G₁ or early DNA synthetic (S) phases. Markedly different growth patterns resulted from irradiation in different phases. Prolongation of interphase was minimal when cells were irradiated early in G₁ and rose progressively through the remainder of the cycle. Cells irradiated while in mitosis were not delayed in that division, but the succeeding division was delayed. Persistence of cells as metabolizing entities did not depend on the phase of the division cycle in which they are irradiated. Characteristic perturbations of the normal DNA synthetic cycle occurred. Cells irradiated in M suffered a small delay in the onset of S, a slight prolongation of S, and a slight depression in the rate of DNA synthesis, and the major delay occurred in G₂. Cells irradiated in G₁ showed no delay in the onset of S, and essentially no alteration in the duration or rate of DNA synthesis, and G₂ delay was minimal. Cells irradiated in S suffered an appreciable S prolongation and a decreased rate of DNA synthesis. G₂ delay was shorter than S delay. (auth)

• Research Organization: Mallinckrodt Inst. of Radiology, St. Louis; and Washington Univ., St. Louis
• NSA Number: NSA-17-021493
• OSTI ID: 4719026
• Journal Information: Biophysical Journal (U.S.), Journal Name: Biophysical Journal (U.S.) Vol. Vol: 3; ISSN BIOJA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOCHEMISTRY
CANCER
HELA CELLS
MITOSIS
NUCLEIC ACIDS
POPULATIONS
RADIATION EFFECTS
X RADIATION