TRITIUM LABELING, SIMPLE AND SAFE

Journal Article · Thu Mar 01 04:00:00 EST 1962 · Jewish Mem. Hosp. Bull.

OSTI ID:4711581

Brodie, S S

A modified Wilzbach procedure is described, as well as an apparatus for the preparation of uranium tritide from U metal and T gas. U metal turnings (6 to 8 mesh) are allowed to react with H at 150 c- C, the system is evacuated, and the uranium hydride is heated to 300 to 350 c- C. The liberated H is pumped off and the system refilled with T gas, the temperature being kept below 400 c- C before being reduced to that of room temperature. By subsequent heating of the uranium tritide thus formed, T can be made available for labeling tracer metabolites by the Wilzbach method. A Tesla coil operated at 15000 to 25000 v is used to provide an arc in the reaction chamber where the labeling is usually completed in 5 to 10 min. The unused T and replaced H are resorbed by U metal. Some labeled compounds need further purification, which may be effected by chromatography, ion exchange, electrophoresis, crystallization, or solvent extraction. Compounds successfully prepared by the method are: ACTH, DOCA, hypoxanthine, folic acid, methotrexate, thymine, purines, and amino acids. The yields ranged from 30 C to 3 mC/mg, depending upon the number of nonlabile H positions that are available. Compounds with sugars attached, such as thymidine, are not amenable to the procedure. Tritiated water, catalysts, and heat are used to prepare the compounds. It was found that water-soluble compounds, tritiated at a double bond, are best preserved by adding Na/sub 2/SO/sub 3/ or weak (NH/sub 4/)/sub 2/S. For compounds soluble in nonaqueous solvents, some antioxidants used by rubber companies proved valuable. (TCO)

• Research Organization: Jewish Memorial Hospital, New York
• NSA Number: NSA-17-025298
• OSTI ID: 4711581
• Journal Information: Jewish Mem. Hosp. Bull., Journal Name: Jewish Mem. Hosp. Bull. Vol. Vol: 6-7
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ACTH
AMINO ACIDS
AMMONIUM COMPOUNDS
ANTIMITOTIC DRUGS
BINDING ENERGY
CATALYSIS
CHEMISTRY
CHROMATOGRAPHY
COILS
CORTISONE
CRYSTALLIZATION
DEGASSING
DRUGS
ELECTROPHORESIS
FOLIC ACID
HEATING
HETEROCYCLICS
HORMONES
HYDROGEN
HYPOXANTHINE
IMPURITIES
ION EXCHANGE
KETONES
LABELLED COMPOUNDS
METEORITES
METHOTREXATE
MINERAL ACIDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PREPARATION
PURINES
PYRIDINES
PYRIMIDINES
RUBBER
SODIUM COMPOUNDS
SOLUBILITY
SOLVENT EXTRACTION
STEROIDS
SUGARS
SULFIDES
SULFUROUS ACID
TEMPERATURE
THYMIDINE
THYMINE
TRITIUM
URANIUM
URANIUM HYDRIDES
VITAMIN B GROUP
VITAMINS
WILZBAC