STUDIES WITH PURPLE ADENINE MUTANTS IN NEUROSPORA CRASSA. V. EVIDENCE FOR ALLELIC COMPLEMENTATION AMONG ad-3B MUTANTS
Journal Article
·
· Genetics (U.S.)
OSTI ID:4703561
Heterokaryon tests of all possible combinations of 161 ad-3B mutants of independent origin were made in duplicate in test tubes containing liquid minimal medium. Only 112 (0.4 percent) of the 25,760 pairwise combinations involving different mutants were positive. All combinations showing positive tests involved one of four leaky mutants and 14 nonleaky mutants. Growth tube studies on the heterokaryons revealed a wide range of growth rates from wild type to oniy slightly faster than the rate of leaky mutant itself. These tests show that the complementation map of the ad-3B cistron consists of at least two complementation subgroups or complons. (auth)
- Research Organization:
- Oak Ridge National Lab., Tenn.
- NSA Number:
- NSA-17-019764
- OSTI ID:
- 4703561
- Journal Information:
- Genetics (U.S.), Journal Name: Genetics (U.S.) Vol. Vol: 48; ISSN GENTA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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