Single-molecule spectroscopy and dynamics at room temperature

Journal Article · Sun Dec 01 04:00:00 EST 1996 · Accounts of Chemical Research

DOI:https://doi.org/10.1021/ar950246m· OSTI ID:468734

Xie, X S ^[1]

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1. Pacific Northwest National Lab., Richland, WA (United States)

The spirit of studying single-molecule behaviors dates back to the turn of the century. In addition to Einstein`s well-known work on Brownian motion, there has been a tradition for studying single {open_quotes}macromolecules{close_quotes} or a small number of molecules either by light scattering or by fluorescence using an optical microscope. Modern computers have allowed detailed studies of single-molecule behaviors in condensed media through molecular dynamics simulations. Optical spectroscopy offers a wealth of information on the structure, interaction, and dynamics of molecular species. With the motivation of removing {open_quotes}inhomogeneous broadening{close_quotes}, spectroscopic techniques have evolved from spectral hole burning, fluorescence line narrowing, and photo-echo to the recent pioneering work on single-molecule spectroscopy in solids at cryogenic temperatures. High-resolution spectroscopic work on single molecules relies on zero phonon lines which appear at cryogenic temperatures, and have narrow line widths and large absorption cross sections. Recent advances in near-field and confocal fluorescence have allowed not only fluorescence imaging of single molecules with high spatial resolutions but also single-molecule spectroscopy at room temperature. In this Account, the author provides a physical chemist`s perspective on experimental and theoretical developments on room-temperature single-molecule spectroscopy and dynamics, with the emphasis on the information obtainable from single-molecule experiments. 61 refs., 9 figs.

• OSTI ID: 468734
• Journal Information: Accounts of Chemical Research, Journal Name: Accounts of Chemical Research Journal Issue: 12 Vol. 29; ISSN 0001-4842; ISSN ACHRE4
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
FLUORESCENCE SPECTROSCOPY
MOLECULES
REVIEWS