Direct measurement of single-molecule diffusion and photodecomposition in free solution
- Ames Lab., IA (United States)
Continuous monitoring of submillisecond free-solution dynamics of individual rhodamine-6G molecules and 30-base single-stranded DNA tagged with rhodamine was achieved. Fluorescence images were recorded from the same set of isolated molecules excited either through the evanescent field at the quartz-liquid interface or as a thin layer of solution defined by micron-sized wires, giving diffraction-limited resolution of interconnected attoliter volume elements. The single-molecule diffusion coefficients were smaller and the unimolecular photodecomposition lifetimes were longer for the dye-DNA covalent complex as compared with those of the dye molecular itself. Unlike bulk studies, stochastic behavior was found for individual molecules of each type, and smaller diffusion coefficients were observed. 19 refs., 4 figs., 1 tab.
- OSTI ID:
- 468288
- Journal Information:
- Science, Vol. 275, Issue 5303; Other Information: PBD: 21 Feb 1997
- Country of Publication:
- United States
- Language:
- English
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