TRANSPORT OF NUCLEIC ACIDS AND PROTEINS THROUGH THE CELLULAR MEMBRANES

Journal Article · Mon Jan 01 04:00:00 EST 1962 · Protides of the Biological Fluids, Proceedings of the Colloquium (Belgium)

OSTI ID:4680540

Ledoux, L; Galand, P

Experiments in plants and animals with H/sup 3/-labeled macromolecules showed that exogenous ribonucleic acid, deoxyribonucleic acid, and ovalbumin can penetrate cell membranes and become deposited in tissues in macromolecular form. The injected macromolecules are taken up particularly by proliferating tissues, as shown by the rapid uptake of intraperitoneally or intravenously injected DNA-H/ sup 3/ by the ovaries and vagina of prepupertal mice treated with estradiol. This phenomenon can also be demonstrated in vitro; cultured ascites tumor cells were found to take up RNA, DNA, and proteins. Irradiation of the cells with 800- r doses of x rays inhibited this incorporation of macromolecules, but not that of metabolites from the culture medium. In norirradiated cells the ingested RNA was selectively taken up by mitochondria, without affecting the respiratory activity of the cells. Injection of RNA-H/sup 3/ intraperitoneally in mice results in the rapid labeling of most organs. (H.H.D.)

• Research Organization: CEN, Mol. Belg.
• NSA Number: NSA-17-024868
• OSTI ID: 4680540
• Journal Information: Protides of the Biological Fluids, Proceedings of the Colloquium (Belgium), Journal Name: Protides of the Biological Fluids, Proceedings of the Colloquium (Belgium) Vol. Vol: 10; ISSN PBFPA
• Country of Publication: Country unknown/Code not available
• Language: French

Related Subjects

ALBUMINS
ANIMAL CELLS
BIOLOGY AND MEDICINE
CYTOLOGY
DIFFUSION
GONADS
HORMONES
METABOLISM
MICE
NUCLEIC ACIDS
OVARIES
PLANT CELLS
PROTEINS
RADIATION DOSES
RIBONUCLEIC ACID
SEX
TRACER TECHNIQUES
TRITIUM
TUMORS
X RADIATION