ANTITOXIN FORMATION BY CELLS TRANSPLANTED TO IRRADIATED ANIMALS
The aim of the work was to study, in conditions of normal subcutaneous immunization, which transplanted cells form antitoxin, the significance of the phase of antibody formation, the immunological response of cells to stimulation by 2 unrelated antigens, and to find whether transplanted cells are capable of a secondary immunological response. Donor animals were immunized subcutaneously: guinea pigs twice, with a 30-day interval, with adsorbed diphtheria toxoid, or once with native toxoid; rabbits simultaneously with 2 antigens, the diphtheria toxoid and typhoid formol vaccine, once or several times over 30 days. Recipient animals were exposed to x rays, guinea pigs to 200 or 525 r, and rabbits to 550 or 800 r, and injected intravenously 2 to 4 hr later with cell suspensions prepared from donor spleen, lymphatic nodes, or bone marrow or, for controls, with cells killed by heating. In 3 trials to study the inductive phase of antibody formation, cells were taken from donors 24 hr after single immunization. In guinea pigs exposed to 200 or 525 r and in rabbits to 550 r, there was no sign of anti-diphtheria antitoxin or, in the rabbits, agglutinins during 30 days after transplantation of cells. In 4 trials of antibody production by cells taken in the reproductive phase, all transplanted tissues formed, in up to 10 days, detectable amounts of antitoxin in guinea pigs, except bone marrow in those exposed to 200 r; killed cells produced none. In rabbits exposed to 550 r, transplanted cells formed antibodies to both antigens used; they appeared by the 3rd day, reached a maximum on the 6th, fell rapidly by the 15th, and were not detectable by the 30th day. The pattern was similar for both antigens. Basically the same results were obtained in rabbits exposed to 800 r. When recipient animals were injected with diphtheria toxoid and formol vaccine on the 15th day afier transplantation, no antitoxin was formed but agglutinin titer rose in all animals, even in those injected with killed cells. (OTS)
- Research Organization:
- Inst. of Epidemiology and Microbiology, Moscow
- NSA Number:
- NSA-17-030402
- OSTI ID:
- 4678143
- Journal Information:
- Byul. Eksperim. Biol. i Med., Vol. Vol: 55; Other Information: Orig. Receipt Date: 31-DEC-63
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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Related Subjects
ABSORPTION
ANTIBODIES
ANTIGENS
ARGON
ATOMIC MODELS
BACTERIA
BLOOD VESSELS
BONE MARROW
CROSS SECTIONS
CYTOLOGY
DIPHTHERIA
ELECTRONS
ENERGY
ENERGY LEVELS
ERRORS
EXCITATION
GUINEA PIGS
HELIUM
IMMUNITY
INFECTIONS
IONIZATION
KRYPTON
LYMPH SYSTEM
MEASURED VALUES
NEON
OSCILLATOR STRENGTH
PHOTOELECTRIC EFFECT
PROTEINS
QUANTITY RATIO
RABBITS
RADIATION DOSES
REFRACTION
SKIN
SPLEEN
SUSPENSIONS
TABLES
TOXICITY
TRANSIENTS
TRANSPLANTS
VARIATIONS
X RADIATION
XENON