THE MECHANISM OF THE RADIOBIOLOGICAL LABILIZATION OF HEMOGLOBIN

Journal Article · Tue May 21 04:00:00 EDT 1963 · Doklady Akademii Nauk SSSR (U.S.S.R.) English translation currently published in a number of subject-oriented journals

OSTI ID:4672198

Bauman, E A

globin samples of the blood were modified by treatment with formaldehyde and oximes. The stability of the initial hemoglobin and of the chemically modified forms was estimated from the degradation of the hemoglobin in a buffered tartrate solution. The addition of formaldehyde blocking groups to the hemoglobin taken from irradiated rabbits led to stabilization of the hemoglobin molecule. Blocking with oxime groups was much less effective. Experiments on the recombination of heme molecules with the globin protein molecule showed that heme molecules combined more readily with native globin than with denatured globin. Treatment of the globin with the nitroprusside ion STAFe(cn)/sub 5/NO!/ sup 2-/ or with oximes had very little effect on the recombination of heme molecules with globin, but treatment of the globin with formaldehyde led to a significant increase in the recombination rate. The formaldehyde moleucles react with the basic amine groups in the globin molecule, but leave the carboxyl groups free to combine with the heme molecules. (TTT)

• Research Organization: Originating Research Org. not identified
• NSA Number: NSA-17-033662
• OSTI ID: 4672198
• Journal Information: Doklady Akademii Nauk SSSR (U.S.S.R.) English translation currently published in a number of subject-oriented journals, Journal Name: Doklady Akademii Nauk SSSR (U.S.S.R.) English translation currently published in a number of subject-oriented journals Vol. Vol: 150; ISSN DANKA
• Country of Publication: Country unknown/Code not available
• Language: Russian

Related Subjects

ALDEHYDES
AMINES
BIOCHEMISTRY
BIOLOGY AND MEDICINE
BLOOD
CHEMICAL REACTIONS
FORMALDEHYDE
FORMIC ACID
GLOBIN
HEME
HEMOGLOBIN
ORGANIC NITROGEN COMPOUNDS
OXIMES
PROTEINS
RABBITS
RADIATION EFFECTS
X RADIATION