Detection of obesity QTLs on mouse chromosomes 1 and 7 by selective DNA pooling
- Jackson Lab., Bar Harbor, ME (United States)
The inheritance of obesity has been analyzed in an intercross between the lean 129/Sv mouse strain and the obesity-prone EL/Suz mouse strain. The weights of three major fat pads were determined on 4-month-old mice, and the sum of these weights, divided by body weight, was used as an adiposity index. The strategy of selective DNA pooling was used as a primary screen to identify putative quantitative trait loci (QTLs) affecting adiposity index. DNA pools representing the leanest 15% and fattest 15% of the F2 progeny were compared for differential allelic enrichment using widely dispersed microsatellite variants. To evaluate putative QTLs, individual genotyping and interval mapping were employed to estimate QTL effects and assess statistical significance. One QTL affecting adiposity index, which accounted for 12.3% of phenotypic variance in gender-merged data, was mapped to the central region of Chromosome (Chr) 7. The QTL allele inherited from EL conferred increased adiposity. A second QTL that accounts for 6.3% of phenotypic variance was identified on Chr 1 near D1Mitt211. At both QTLs, the data are consistent with dominant inheritance of the allele contributing to obesity. The possible relationships between these QTLs and previously described obesity QYLs, major obesity mutations, and candidate genes are discussed. 42 refs., 3 figs., 3 tabs.
- OSTI ID:
- 466688
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 34; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
Similar Records
Genetic modifiers of Lepr{sup fa} associated with variability in insulin production and susceptibility to NIDDM
Mapping the mouse dactylaplasia mutation, Dac, and a gene that controls its expression, mdac
Genetic analysis in the Collaborative Cross breeding population
Journal Article
·
Thu May 01 00:00:00 EDT 1997
· Genomics
·
OSTI ID:530728
Mapping the mouse dactylaplasia mutation, Dac, and a gene that controls its expression, mdac
Journal Article
·
Wed Sep 20 00:00:00 EDT 1995
· Genomics
·
OSTI ID:258285
Genetic analysis in the Collaborative Cross breeding population
Journal Article
·
Fri Dec 31 23:00:00 EST 2010
· Genome Research
·
OSTI ID:1034663