Cell transfection as a tool to study growth hormone action

Journal Article · Sat Dec 31 04:00:00 EST 1994 · Proceedings of the Society for Experimental Biology and Medicine

DOI:https://doi.org/10.3181/00379727-206-43738· OSTI ID:466612

Norstedt, G; Enberg, B; Francis, S ^[1]

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1. Karolinska Institute, Huddinge (Sweden); and others

The isolation of growth hormone receptor (GHR) cDNA clones has made possible the transfection of GHRs into cultured cells. Our aim in this minireview is to show how the application of such approaches have benefited GHR research. GH stimulation of cells expressing GHR cDNAs can cause an alteration of cellular function that mimic those of the endogenous GHR. GHR cDNA transfected cells also offer a system where the mechanism of GH action can be studied. Such a system has been used to demonstrate that the GHR itself becomes tyrosine phosphorylated and that further phosphorylation of downstream proteins is important in GH action. The GH signals are transmitted to the nucleus and GH regulated genes have now begun to be characterized. The ability to use cell transfection for mechanistic studies of GH action will be instrumental to define domains within the receptor that are of functional importance and to determined pathways whereby GH signals are conveyed within the cell. 33 refs., 2 tabs.

• Sponsoring Organization: USDOE
• OSTI ID: 466612
• Journal Information: Proceedings of the Society for Experimental Biology and Medicine, Journal Name: Proceedings of the Society for Experimental Biology and Medicine Vol. 206; ISSN PSEBAA; ISSN 0037-9727
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ANIMAL CELLS
DNA-CLONING
FUNCTIONAL MODELS
GENE REGULATION
PHOSPHORYLATION
PROTEINS
RECEPTORS
REVIEWS
STH
STRUCTURE-ACTIVITY RELATIONSHIPS
TYROSINE