Isolation, expression, and chromosomal localization of the human mitochondrial capsule selenoprotein gene (MCSP)
- Institut fuer Humangenetik der Universitaet, Goettingen (Germany); and others
The mitochondrial capsule selenoprotein (MCS) (HGMW-approved symbol MCSP) is one of three proteins that are important for the maintenance and stabilization of the crescent structure of the sperm mitochondria. We describe here the isolation of a cDNA, the exon-intron organization, the expression, and the chromosomal localization of the human MCS gene. Nucleotide sequence analysis of the human and mouse MCS cDNAs reveals that the 5{prime}- and 3{prime}-untranslated sequences are more conserved (71%) than the coding sequences (59%). The open reading frame encodes a 116-amino-acid protein and lacks the UGA codons, which have been reported to encode the selenocysteines in the N-terminal of the deduced mouse protein. The deduced human protein shows a low degree of amino acid sequence identity to the mouse protein. The deduced human protein shows a low degree of amino acid sequence identity to the mouse protein (39%). The most striking homology lies in the dicysteine motifs. Northern and Southern zooblot analyses reveal that the MCS gene in human, baboon, and bovine is more conserved than its counterparts in mouse and rat. The single intron in the human MCS gene is approximately 6 kb and interrupts the 5{prime}-untranslated region at a position equivalent to that in the mouse and rat genes. Northern blot and in situ hybridization experiments demonstrate that the expression of the human MCS gene is restricted to haploid spermatids. The human gene was assigned to q21 of chromosome 1. 30 refs., 9 figs.
- OSTI ID:
- 466591
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 32; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
AMINO ACID SEQUENCE
CODONS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
EXONS
GENE REGULATION
GENETIC EFFECTS
GENETIC MAPPING
HAPLOIDY
HUMAN CHROMOSOME 1
INTRONS
MICE
MITOCHONDRIA
NUCLEOTIDES
POLYMERASE CHAIN REACTION
PROTEINS
SELENIUM
SPERMATOZOA
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION